Fvs780 apc-cy7
Webproperties, APC-Cy7 and APC-H7 cannot be used simultaneously. •••••• 633 nm 635 nm 640 nm 780/60 APC-H7 (Ex max 650 nm/Em max 785 nm) is an APC-cyanine tandem fluorochrome which uses an analog of Cy7 and has similar spectral properties to APC-Cy7. APC-H7 conjugates provide greater stability in light and paraformaldehyde WebThis tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission …
Fvs780 apc-cy7
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WebAcquired cell suspension was filtered, lysed with ACK Lysis Buffer, stained with FVS780- APC-CY7 and Human Fc block. Afterwards, cells were stained with anti-human antibodies for 10min at 4°C: Lineage Cocktail-Pacific Blue (BD, 562722, 1:100), CD45- APC-CY7 (BD, 557833, 1:100), E-Cadherin-BV421 (BD, 743712, 1:100), CD31-PE-CY7 (BD, 563651, 1: ...
WebAPC-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. APC-Cy7 is a tandem fluorochrome composed of … WebAPC/Cyanine7 anti-mouse CD45 Antibody - CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. Products; Learn; Support; ... BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance …
WebAPC-Cy7, or CD8 APC-Cy7. Stained samples were stored in 1% PFA at room temperature under ambient light for 48 hours. BD APC-H7 vs. APC-Cy7: Comparison of Staining Stability (in BDSF, under light at RT) 0 50 100 150 200 250 0 5 10 15 20 25 30 35 40 45 50 CD4 APC-H7 Time (hour) CD8 APC-H7 WebMay 17, 2024 · The spillover of APCCy7 to APC in FACS. I have 10-colour panel with following fluorochromes PerCPCy5-5, FITC, BV785, BV605, BV421, DAPI, APC-Cy7, Alexa Fluor 700, APC, PE-Cy7. In single colour ...
WebJul 18, 2024 · Prepare FVS780 working solution by adding 28 μL DPBS(-) to a 2 μL FVS780 stock solution (dilute it 1:15). Note: Discard the remaining FVS780 stock solution once thawed. Add 3 μL of FVS780 working solution to 100 μL TIL suspension, mix thoroughly by pipetting, and incubate for 10 min, protected from light.
WebAcquired cell suspension was filtered, lysed with ACK Lysis Buffer, stained with FVS780- APC-CY7 and Human Fc block. Afterwards, cells were stained with anti-human … chance the rapper brain cells lyricsWebJul 5, 2024 · E-mail (s) [email protected]. Organization name. Ninth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine. Street address. … chance the rapper – child of god mp3 downloadWeb0 or APC-Alex nsation, it is re age of dead ce em at 65°C fo be combined at compensatio <5%. Actual c settings. igh-quality, an e freeze-thaw lysis. ouse thymocyt e Foxp3/Trans … harbor freight dust collector reviewsWebBring FVS780 dye powder and 180 μl of fresh cell culture-grade Dimethyl Sulfoxide (DMSO; eg, Sigma D2650) to room temperature. ... can be … chance the rapper - child of god 2022WebAbout PE-Cy7. PE-Cyanine®7 (PE-Cy7, RPE-Cy7) is a far red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy7. The donor molecule, PE can be excited by … chance the rapper - child of godWebAPC-eFluor® 780 (APC-eF780) is a red-emitting tandem fluorophore that combines allopycocyanin (APC) with eF780. The donor, APC, can be excited by the 640 nm red … chance the rapper cigarWebThe vial of APC/Cy7 or APC/Fire™ 750 was stored in the dark at either 4°C or 37°C for 81 days. At certain intervals, an aliquot of reagent was used to stain Veri-Cells™, a … harbor freight dust mop