Sigma aldrich poly l lysine
WebOct 6, 2024 · Mouse BV-2 cells were cultured in RPMI-1640 medium (R7509, Sigma-Aldrich) supplemented with 2.4 mM L-glutamine (17-605E; Gibco), 10% (v/ ... and the cells were resuspended in the culture medium and plated for experiments on poly-d-lysine (P6407, Sigma-Aldrich) coated coverslips or on cell culture plates for maintenance. WebUses Poly-L-Lysine of 84000 d, yielding solution viscosity for easy handling while providing sufficient binding sites for cell attachment; Product is filtered through a 0.2µm filter before freezing; Specifications. Specifications. Content And Storage: Store at -20°C for up to 4 months from date of receipt.
Sigma aldrich poly l lysine
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WebPoly-L-lysine (PLL) and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The senescence detection kit was obtained from BioVision Inc. Oligonucleotides for polymerase chain reaction (PCR), reverse transcription, and cDNA were synthesized by Bioneer (Bioneer Corporation, Daejeon, Korea). WebProduct Name POLY-L-LYSINE SOLUTION BIOREAGENT, STERILE-FILTERED, SUITABLE FOR CELL CULTURE Product Number P4707 Company Sigma-Aldrich GmbH Industriestrasse 25 9470 Buchs Switzerland Technical Phone # 41-81-755-2511 Fax 41-81-756-5449 E-mail Address [email protected] Emergency Phone ...
WebPOLY-L-LYSINE SOLUTION - Find MSDS or SDS, a COA, data sheets and more information. ... P8920-500ML Sigma-Aldrich POLY-L-LYSINE SOLUTION More>> Less<< P8920-500ML 0 Retrieving price... Price could not be retrieved Minimum Quantity is a … WebThe following protocol applies to product numbers P4707*, P4832*, P7280, P6407, P7405, P9155, P6282, and P5899. Optimal conditions must be determined for each cell line and application. Add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine. Aseptically coat culture surface with 1.0 mL/25 cm 2 (only).
WebPoly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. Certain cell types secrete proteases, which can digest Poly-L-Lysine. The molecular weight of Poly-L-Lysine ... WebPoly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O …
WebSIGMA-ALDRICH sigma-aldrich.com Material Safety Data Sheet Version 3.6 Revision Date 04/26/2012 Print Date 03/19/2013 1. PRODUCT AND COMPANY IDENTIFICATION Product name : Poly-L-lysine hydrobromide Product Number : P2636 Brand : Sigma Supplier : Sigma-Aldrich 3050 Spruce Street SAINT LOUIS MO 63103 USA Telephone : +1 800-325-5832 …
WebPoly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine. This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm 2. simple food dogWebPoly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O … simple food flyerWebSigma Aldrich Fine Chemicals Biosciences Poly-L-lysine solution, 25988-63-0, MFCD00165424, 50 mL. Molecular Formula: C18 H36 N6 O3 X2 . 3 Br H, Molecular Weight: 627.257. Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, … simple food drawingsWebDescription. Poly-D-Lysine solution, 1.0 mg/mL. Overview. Poly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da. raw is bestWebWith cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor. Components. Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ... simple food for campingWebMar 25, 2024 · Cell proliferation was assessed by colorimetric assay based on 3-(4.5-dimethyl thiazol 2-y1)-2.5-diphenyl tetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MO, USA) metabolization, according to the protocol optimized by Mosmann . Cells were seeded on poly-l-Lysine-coated 96-well plates at a density of 1.2 × 10 4 cells/well. simple food for bridal showerWebSigma-Aldrich. P6282. Poly-L-lysine hydrobromide. mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture rawish western market